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anti at180  (Novus Biologicals)


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    Novus Biologicals anti at180
    Anti At180, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+at180/pm40653030-102-36-39?v=Novus+Biologicals
    Average 94 stars, based on 7 article reviews
    anti at180 - by Bioz Stars, 2026-08
    94/100 stars

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    Image Search Results


    CBD differentially affects the phosphorylation of AT8, AT180 proteins in EGFP-Tau WT and EGFP-Tau-PL301-expressing cell lines. EGFP-Tau WT cells were treated with CBD (100, 250 nM, 1 and 10 µM) for 24 h and cytosolic levels of AT8, AT180 and total tau (Tau-5) were evaluated by western blotting. EGFP-Tau-P301L cells were treated with CBD, ACEA and GW405833 at 1 µM for 24 h and cytosolic levels of AT8 were evaluated by western blotting. Data represent the mean obtained from the quantification of the optical density of the bands obtained by western blotting with the antibodies ( A ) anti-tau-5 (70-100 kD), ( B ) anti-pAT180 (70-100 kD), ( C - D ) anti-p-AT8 (70-100 kD) and normalized to the internal control α-tubulin (50 kD) or GAPDH (36 kD), as shown in the histograms (mean ± SEM). * p <0.05 and **** p <0.0001 vs. CTR (Dox – ) and * p <0.05, *** p <0.001 and **** p <0.0001 vs. CTR (Dox + ) ( n =3-5; One-way ANOVA, followed by the Sidak post-test)

    Journal: Neurotoxicity Research

    Article Title: Cannabidiol-Induced Autophagy Ameliorates Tau Protein Clearance

    doi: 10.1007/s12640-025-00729-3

    Figure Lengend Snippet: CBD differentially affects the phosphorylation of AT8, AT180 proteins in EGFP-Tau WT and EGFP-Tau-PL301-expressing cell lines. EGFP-Tau WT cells were treated with CBD (100, 250 nM, 1 and 10 µM) for 24 h and cytosolic levels of AT8, AT180 and total tau (Tau-5) were evaluated by western blotting. EGFP-Tau-P301L cells were treated with CBD, ACEA and GW405833 at 1 µM for 24 h and cytosolic levels of AT8 were evaluated by western blotting. Data represent the mean obtained from the quantification of the optical density of the bands obtained by western blotting with the antibodies ( A ) anti-tau-5 (70-100 kD), ( B ) anti-pAT180 (70-100 kD), ( C - D ) anti-p-AT8 (70-100 kD) and normalized to the internal control α-tubulin (50 kD) or GAPDH (36 kD), as shown in the histograms (mean ± SEM). * p <0.05 and **** p <0.0001 vs. CTR (Dox – ) and * p <0.05, *** p <0.001 and **** p <0.0001 vs. CTR (Dox + ) ( n =3-5; One-way ANOVA, followed by the Sidak post-test)

    Article Snippet: Membranes were incubated with primary antibodies overnight at 4 °C: anti-LC3B (Cell Signaling Technology Inc., #2775S, 1:2000,), anti-p-AT8-tau (pSer202/pTrh205, Thermo Fisher Scientific, #MN1020, 1:1000), anti-p-AT180-tau (Trh231, Thermo Fisher Scientific, #MN1040, 1:1000), anti-tau-5 (Abcam, #ab80579, 1:1000) and anti-p62 (MBL #PM045, 1:2000), α-tubulin (Sigma-Aldrich, #T8203, 1:5000) or GAPDH (Sigma-Aldrich, #G8795, 1:5000) as housekeep control.

    Techniques: Expressing, Western Blot, Control